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The IDT Primer Calculator Tool calculates primer concentration using the basic formula: Concentration = Mass / Volume. It helps researchers quickly determine the concentration of their IDT primers for molecular biology experiments.
The calculator uses the concentration formula:
Where:
Explanation: The formula calculates concentration by dividing the mass of the primer by the volume of the solution it's dissolved in.
Details: Accurate primer concentration calculation is essential for PCR experiments, sequencing reactions, and other molecular biology techniques that require precise primer quantities for optimal results.
Tips: Enter mass in nanograms (ng) and volume in microliters (µl). Both values must be positive numbers greater than zero for accurate calculation.
Q1: Why is primer concentration important?
A: Proper primer concentration ensures optimal annealing in PCR reactions, affects specificity and yield, and is critical for successful amplification.
Q2: What are typical concentration ranges for primers?
A: Most PCR reactions use primers at concentrations between 0.1-1.0 µM, which corresponds to approximately 6-60 ng/µl for a 20-base primer.
Q3: How should I prepare my primer stock solution?
A: Resuspend lyophilized primers in nuclease-free water or TE buffer according to the manufacturer's instructions, then calculate the concentration using this tool.
Q4: Can I use this calculator for other oligonucleotides?
A: Yes, this calculator works for any oligonucleotide where you need to calculate concentration from mass and volume.
Q5: What if my primer concentration is too high or too low?
A: Adjust by either adding more solvent to dilute a concentrated solution or by adding more primer to increase concentration in a dilute solution.