1. What is Oligo Concentration Calculation?

The oligo concentration calculation determines the concentration of oligonucleotides using the basic formula: Concentration = Mass / Volume. This is essential for molecular biology experiments requiring precise oligonucleotide concentrations.

2. How Does the Calculator Work?

The calculator uses the simple formula:

Where:

• \( Mass \) — Mass of oligonucleotide in nanograms (ng)
• \( Volume \) — Volume of solvent in microliters (µl)

Explanation: This straightforward calculation provides the concentration in ng/µl, which is commonly used in laboratory settings for oligonucleotide preparations.

3. Importance of Concentration Calculation

Details: Accurate concentration measurement is crucial for PCR, sequencing, and other molecular biology techniques that require precise oligonucleotide quantities for optimal results.

4. Using the Calculator

Tips: Enter mass in nanograms (ng) and volume in microliters (µl). Both values must be positive numbers greater than zero for accurate calculation.

5. Frequently Asked Questions (FAQ)

Q1: Why is concentration measured in ng/µl?
A: ng/µl is a convenient unit for oligonucleotide concentration as it provides a practical measurement for laboratory pipetting and dilution calculations.

Q2: What is a typical oligo concentration range?
A: Typical concentrations range from 10-100 ng/µl, depending on the synthesis scale and application requirements.

Q3: How should I measure mass accurately?
A: Use a precision balance for dry oligo measurement or spectrophotometric methods for dissolved oligo quantification.

Q4: Can I convert ng/µl to other units?
A: Yes, ng/µl can be converted to µg/µl (divide by 1000) or mg/ml (divide by 1,000,000) as needed.

Q5: What if my oligo is in different units?
A: Ensure consistent units - convert all measurements to ng and µl before using this calculator for accurate results.