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Protein Coefficient Equation:
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The protein coefficient (also known as molar extinction coefficient) is a measure of how strongly a chemical species absorbs light at a given wavelength. It is a fundamental parameter in spectrophotometry for quantifying protein concentrations.
The calculator uses the protein coefficient equation:
Where:
Explanation: The equation calculates the molar absorptivity coefficient, which represents the absorbance of a solution per unit concentration and path length.
Details: Accurate determination of protein coefficients is essential for protein quantification, purity assessment, and various biochemical analyses in research and clinical laboratories.
Tips: Enter absorbance (unitless), concentration in mol/L, and path length in cm. All values must be valid positive numbers.
Q1: What is the typical range for protein coefficients?
A: Protein coefficients typically range from 10,000 to 100,000 L/mol·cm, depending on the specific protein and wavelength used.
Q2: Why is the path length important in this calculation?
A: Path length directly affects absorbance measurements. Standard cuvettes typically have a 1 cm path length, which is the reference standard.
Q3: Can this calculator be used for any wavelength?
A: Yes, but the coefficient is wavelength-specific. The calculated value applies only to the wavelength at which the absorbance was measured.
Q4: What are common sources of error in this calculation?
A: Common errors include inaccurate concentration measurements, non-linear absorbance responses, and deviations from Beer-Lambert law assumptions.
Q5: How is this coefficient used in practical applications?
A: It's used to determine unknown concentrations of proteins from absorbance measurements and to characterize protein properties in solution.